Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). These properties were analyzed both in vitro and applied in vivo to neural tissue specimen. 10 The patch formation of Alexa 488-labeled CTX and gangliosides complex represents the clusters of LRs. tdTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. 2008, 26 (10): 527-530. Tetramethyl Rhodamine Isothiocyanate (TRITC) 557 576 EviTag TM quantum dots-Birch Yellow 560 580 Cy3.5® 576 589 568,543 Rhodamine Red-X 570 590 PE-Dyomics®590 488 599 EviTag TM quantum dots-Fort Orange 585 600 ROX 575 602 Alexa Fluor®568 578 603 532 Red 613 480,565 613 Texas Red ® 595 613 568,543,514 HiLyte FluorTM 594 593 616 PE-Texas Red ® 566 616 Alexa Fluor®594 590 617 … For Red excitation, Texas Red is not a good choice because we do not have HeNe 590 nm line, Kr 568 excites it only 35% while HeNe 633 excites it at a negligible level. There is no discrete line in the optimal range for FITC excitation. 2. At room temperature, a sample of purified protein initially shows a major component of green fluorescence (ex/em 475/499 nm), which peaks in intensity around 7 h and decreases to nearly zero over 2 days. Common filter blocks are named after the type of excitation filter: UV or U (Ultraviolet excitation for dyes such as DAPI and Hoechst 33342), B (Blue excitation for FITC and related dyes), and G (Green excitation for TRITC, Texas Red®, etc.). It is reported to be a somewhat slowly … It is commonly conjugated to antibodies and proteins for cellular imaging applications. These shifts conform to the somewhat higher wavelength ranges for the Texas Red (green excitation; red emission) bands. Instead, fluorophores with absorption peaks residing at longer wavelengths, such as Alexa Fluor 568, Alexa Fluor 594, or Texas Red (578, 590, and 596-nanometer absorption maxima, respectively) should be employed with the krypton-argon laser. Alexa Fluor ® 647 650 Red FAM 495 520 Green FITC 495 528 Green (Fluorescein Isothiocyanate) PE (Phycoerythrin) 496 and 545/566 576 Orange-yellow SureLight® P1 545 666 Red SureLight® P3 614 662 Red SureLight® PE 480 578 Yellow Spectral Red (SPRD) (PE/Cy5 ®) 565 666 Red Texas Red ® 490,675 695 Red TRITC 557 576 Orange-yellow The excitation spectrum was scanned while measuring emission at 650 nm, and the emission spectrum was scanned while exciting at 500 nm. The Texas Red light cube provides a 585/29 Ex and 624/40 Em filter set. They are fabricated at industry-standard … TRITC, Texas red, phycoerythrin, Cy3: BP 546: FT 580: LP 590: 487915: AMCA: BP 365: FT 395: LP 397: 487901: FITC and DTAF give green fluorescence; phycoerythrin gives orange, and AMCA gives blue. These red-emitting dyes can be efficiently excited using an arc lamp with a strong peak in the 550 nm region and another at 580 nm. This typically would include a nuclear marker such as DAPI, a green-emitting fluorophore such as FITC or GFP, and a red-emitting fluorophore such as mCherry, TRITC, or Texas Red. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used: • in alkaline phosphatase staining in organ sections • for staining chondrogenic spheroids in immunohistochemistry studies • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections. Trends Biotechnol. Anticorps, Protéines recombinantes, coffret ELISA, RNAi, clones ADNc, Antibody Array, coffret Luminex. There is no discrete line in the optimal range for FITC excitation. - TRITC - Texas Red - mCherry - Cyanine (CY3.5) Excitation and Emission Filters are Mounted in Black-Anodized Housings; Dichroic Filters are Unmounted ; Filter Sets are Available at a Savings Over Individual Filter Prices; These excitation, emission, and dichroic filters are designed specifically for use in fluorescence imaging applications. Atto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. Find out how fluorescence microscopes support your research. 10.1016/j.tibtech.2008.06.008. Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. DsRed surprisingly takes days at room temperature to reach full red fluorescence. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum. FITC-Texas Red - The FITC-Texas Red filter combination is designed with slightly different bandpass regions for blue excitation and green emission when compared to the FITC-TRITC set, allowing more efficient excitation of FITC. Excitation (ex) and emission (em) of generally used fluorochromes in order of their ex wavelength. TRITC, Texas red, phycoerythrin, Cy3: BP 546: FT 580: LP 590: 487915: AMCA: BP 365: FT 395: LP 397: 487901: FITC and DTAF give green fluorescence; phycoerythrin gives orange, and AMCA gives blue. Many of the classical fluorescent probes that have been successfully utilized for many years in widefield fluorescence, including fluorescein isothiocyanate (see Figure 1(a), Figure 2, and Figure 3), Lissamine rhodamine, and Texas red (structure illustrated in Figure 1(c); spectra in Figure 2), are also useful in confocal microscopy. 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